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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    WB analysis of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATP1B1 Polyclonal antibody (15192-1-AP)

    WB analysis using 15192-1-AP (same clone as 15192-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    WB analysis of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    WB analysis of mouse heart using 15192-1-AP (same clone as 15192-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    WB analysis of human heart using 15192-1-AP (same clone as 15192-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    IP experiment of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

    IP result of anti-ATP1B1 (IP:15192-1-AP, 4ug; Detection:15192-1-AP 1:2000) with mouse brain tissue lysate 1600 ug. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    IHC staining of human skeletal muscle using 15192-1-AP (same clone as 15192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ATP1B1 Polyclonal antibody (15192-1-AP)

    IHC staining of human skeletal muscle using 15192-1-AP (same clone as 15192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1B1 Polyclonal antibody (15192-1-AP)

    IF Staining of HEK-293 using 15192-1-AP (same clone as 15192-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 15192-1-AP (ATP1B1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15192-1-PBS targets ATP1B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1B1 fusion protein Ag7279 相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, beta 1 polypeptide
Calculated molecular weight 35 kDa
Observed molecular weight 45-52 kDa
GenBank accession numberBC000006
Gene Symbol ATP1B1
Gene ID (NCBI) 481
RRIDAB_2290040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05026
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)