Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
WB analysis using 15192-1-AP (same clone as 15192-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart using 15192-1-AP (same clone as 15192-1-PBS)
mouse heart tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 15192-1-AP (same clone as 15192-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 15192-1-AP (same clone as 15192-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)
IP result of anti-ATP1B1 (IP:15192-1-AP, 4ug; Detection:15192-1-AP 1:2000) with mouse brain tissue lysate 1600 ug. This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 15192-1-AP (same clone as 15192-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 15192-1-AP (same clone as 15192-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 15192-1-AP (same clone as 15192-1-PBS)
Immunofluorescent analysis of HEK-293 cells using 15192-1-AP (ATP1B1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15192-1-PBS in a different storage buffer formulation.

ATP1B1 Polyclonal antibody, PBS Only

ATP1B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15192-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15192-1-PBS targets ATP1B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7279 Product name: Recombinant human ATP1B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 59-303 aa of BC000006 Sequence: LTISEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPNVLPVQCTGKRDEDKDKVGNVEYFGLGNSPGFPLQYYPYYGKLLQPKYLQPLLAVQFTNLTMDTEIRIECKAYGENIGYSEKDRFQGRFDVKIEVKS 相同性解析による交差性が予測される生物種
Full Name	ATPase, Na+/K+ transporting, beta 1 polypeptide
Calculated molecular weight	35 kDa
Observed molecular weight	45-52 kDa
GenBank accession number	BC000006
Gene Symbol	ATP1B1
Gene ID (NCBI)	481
RRID	AB_2290040
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05026
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

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Conjugate

Validation Data Gallery

WB analysis of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

WB analysis using 15192-1-AP (same clone as 15192-1-PBS)

WB analysis of mouse heart using 15192-1-AP (same clone as 15192-1-PBS)

WB analysis of human brain using 15192-1-AP (same clone as 15192-1-PBS)

WB analysis of human heart using 15192-1-AP (same clone as 15192-1-PBS)

IP experiment of mouse brain using 15192-1-AP (same clone as 15192-1-PBS)

IHC staining of human brain using 15192-1-AP (same clone as 15192-1-PBS)