Validation Data Gallery
Tested Applications
| Positive WB detected in | 37°C incubated mouse brain tissue, 37°C incubated rat brain tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84999-4-RR targets ATP1A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ATP1A2 fusion protein Ag10489 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Calculated molecular weight | 1020 aa, 112 kDa |
| Observed molecular weight | 97-100 kDa |
| GenBank accession number | BC052271 |
| Gene Symbol | ATP1A2 |
| Gene ID (NCBI) | 477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50993 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4. The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. For optimal WB detection with ATP1A2, we do not recommend boiling the sample after lysis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP1A2 antibody 84999-4-RR | Download protocol |
| IHC protocol for ATP1A2 antibody 84999-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |