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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant monoclonal antibody (83191-5-RR)

    IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant monoclonal antibody (83191-5-RR)

    IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using ATP1A1 Recombinant monoclonal antibody (83191-5-RR)

    FC experiment of HEK-293 using 83191-5-RR (same clone as 83191-5-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83191-5-RR

    Affinity and Kinetic Characterization of 83191-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83191-5-RR against Human ATP1A1 were performed. The affinity constant is 87.7 pM.

ATP1A1 Recombinant monoclonal antibody, PBS Only

ATP1A1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83191-5-PBS
Clone No.240160B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240160B5, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83191-5-PBS targets ATP1A1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5763

Product name: Recombinant human ATP1A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-681 aa of BC050359

Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI

相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
GenBank accession numberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP05023
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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