Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 21215-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 21215-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 21215-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21215-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 21215-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21215-1-AP (ATOH1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 21215-1-AP
Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21215-1-AP (ATOH1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 21215-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 21215-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
IF Staining of HepG2 using 21215-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21215-1-AP (ATOH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 21215-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ATOH1 (21215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

ATOH1 Polyclonal antibody

ATOH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 21215-1-AP

Publications(50)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), chIP, ELISA

Synonyms

ATH1, bHLHa14, Class A basic helix-loop-helix protein 14, HATH1, Helix-loop-helix protein hATH-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (50)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Neuro-2a cells, HepG2 cells, mouse small intestine tissue, mouse brain tissue, rat brain tissue, C6 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 23 publications below
IHC	See 4 publications below
IF	See 22 publications below
ChIP	See 1 publications below

Product Information

21215-1-AP targets ATOH1 in WB, IHC, IF/ICC, FC (Intra), chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15615 Product name: Recombinant human ATOH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-354 aa of BC069594 Sequence: MSRLLHAEEWAEVKELGDHHRQPQPHHLPQPPPPPQPPATLQAREHPVYPPELSLLDSTDPRAWLAPTLQGICTARAAQYLLHSPELGASEAAAPRDEVDGRGELVRRSSGGASSSKSPGPVKVREQLCKLKGGVVVDELGCSRQRAPSSKQVNGVQKQRRLAANARERRRMHGLNHAFDQLRNVIPSFNNDKKLSKYETLQMAQIYINALSELLQTPSGGEQPPPPPASCKSDHHHLRTAASYEGGAGNATAAGAQQASGGSQRPTPPGSCRTRFSAPASAGGYSVQLDALHFSTFEDSALTAMMAQKNLSPSLPGSILQPVQEENSKTSPRSHRSDGEFSPHSHYSDSDEAS 相同性解析による交差性が予測される生物種
Full Name	atonal homolog 1 (Drosophila)
Calculated molecular weight	354 aa, 38 kDa
Observed molecular weight	38-40 kDa
GenBank accession number	BC069594
Gene Symbol	ATOH1
Gene ID (NCBI)	474
RRID	AB_10733126
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92858
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.

Protocols

Product Specific Protocols
IF protocol for ATOH1 antibody 21215-1-AP	Download protocol
IHC protocol for ATOH1 antibody 21215-1-AP	Download protocol
WB protocol for ATOH1 antibody 21215-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Res Decoding the spatiotemporal regulation of transcription factors during human spinal cord development Authors - Yingchao Shi View Article
human	IF	Immunity IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment. Authors - Xun Lin View Article
human	WB	Nat Cell Biol Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. Authors - Donglim Esther Park View Article
mouse	IHC	J Clin Invest Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea. Authors - Patrick J Atkinson View Article
	IF	Dev Cell Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling Authors - Stefanie Lehr View Article
mouse	IF	Proc Natl Acad Sci U S A Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation Authors - Tianli Qin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 21215-1-AP

WB analysis using 21215-1-AP

WB analysis using 21215-1-AP

WB analysis of HepG2 using 21215-1-AP

IHC staining of human colon cancer using 21215-1-AP

IHC staining of human colon cancer using 21215-1-AP

IF Staining of HepG2 using 21215-1-AP

IF Staining of HepG2 using 21215-1-AP

IF Staining of HepG2 using 21215-1-AP

FC experiment of HepG2 using 21215-1-AP

ATOH1 Polyclonal antibody

ATOH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Decoding the spatiotemporal regulation of transcription factors during human spinal cord development

IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.

Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.

Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.

Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling

Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation