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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATM Recombinant antibody (83608-5-RR)

    WB analysis using 83608-5-RR (same clone as 83608-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83608-5-RR (ATM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ATM Recombinant antibody (83608-5-RR)

    WB analysis of A549 using 83608-5-RR (same clone as 83608-5-PBS)

    WB result of ATM antibody (83608-5-RR; 1:3000; room temperature for 1.5 hours) with wild-type and sg-ATM transfected A549 cells. This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATM Recombinant antibody (83608-5-RR)

    IHC staining of mouse brain using 83608-5-RR (same clone as 83608-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83608-5-RR (ATM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ATM Recombinant antibody (83608-5-RR)

    IF Staining of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATM Recombinant antibody (83608-5-RR)

    IF Staining of HepG2 using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATM Recombinant antibody (83608-5-RR)

    IF Staining of HeLa using 83608-5-RR (same clone as 83608-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATM antibody (83608-5-RR, Clone: 240560F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using ATM Recombinant antibody (83608-5-RR)

    FC experiment of MCF-7 using 83608-5-RR (same clone as 83608-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATM Recombinant antibody (83608-5-RR, Clone:240560F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83608-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83608-5-RR

    Affinity and Kinetic Characterization of 83608-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-5-RR against Human ATM were performed. The affinity constant is 0.318 nM.

  • Featured Product
  • KD/KO Validated

ATM Recombinant monoclonal antibody, PBS Only

ATM Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83608-5-PBS
Clone No.240560F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240560F5, A T mutated, A-T mutated, EC:2.7.11.1, Serine-protein kinase ATM

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83608-5-PBS targets ATM in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag25920

Product name: Recombinant human ATM protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1059-1361 aa of BC137169

Sequence: SSEFENKQALLKRAKEEVGLLREHKIQTNRYTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENSGVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPHHTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRRPQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVDHTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDG

相同性解析による交差性が予測される生物種
Full Name ataxia telangiectasia mutated
Observed molecular weight 310-350 kDa
GenBank accession numberBC137169
Gene Symbol ATM
Gene ID (NCBI) 472
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ13315
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is involved in mitogenic signal transduction, meiotic recombination, detection of DNA damage, and cell cycle control.

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