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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis using 16921-1-AP (same clone as 16921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

    WB result of ATL3 antibody (16921-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATL3 transfected HeLa cells. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis using 16921-1-AP (same clone as 16921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis of HepG2 using 16921-1-AP (same clone as 16921-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SMMC-7721 cells using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis of SMMC-7721 using 16921-1-AP (same clone as 16921-1-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis of mouse liver using 16921-1-AP (same clone as 16921-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ATL3 Polyclonal antibody (16921-1-AP)

    WB analysis of HEK-293 using 16921-1-AP (same clone as 16921-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATL3 Polyclonal antibody (16921-1-AP)

    IP experiment of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

    IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATL3 Polyclonal antibody (16921-1-AP)

    IP experiment of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

    IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:8000) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATL3 Polyclonal antibody (16921-1-AP)

    IHC staining of human stomach cancer using 16921-1-AP (same clone as 16921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATL3 Polyclonal antibody (16921-1-AP)

    IHC staining of rat brain using 16921-1-AP (same clone as 16921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATL3 Polyclonal antibody (16921-1-AP)

    IHC staining of rat brain using 16921-1-AP (same clone as 16921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16921-1-AP (ATL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATL3 Polyclonal antibody (16921-1-AP)

    IF Staining of HeLa using 16921-1-AP (same clone as 16921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (16921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATL3 Polyclonal antibody (16921-1-AP)

    IF Staining of HepG2 using 16921-1-AP (same clone as 16921-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16921-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATL3 Polyclonal antibody, PBS Only

ATL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Atlastin 3, Atlastin-3, EC:3.6.5.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16921-1-PBS targets ATL3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10279

Product name: Recombinant human ATL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC077727

Sequence: MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEAN

相同性解析による交差性が予測される生物種
Full Name atlastin GTPase 3
Calculated molecular weight 541 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC077727
Gene Symbol ATL3
Gene ID (NCBI) 25923
RRIDAB_2290228
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DD88
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

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