Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, fetal human brain tissue, pig brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31924-1-AP targets ATL1 in WB, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29223 Product name: Recombinant human ATL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 449-558 aa of BC010708 Sequence: ATLFVVIFITYVIAGVTGFIGLDIIASLCNMIMGLTLITLCTWAYIRYSGEYRELGAVIDQVAAALWDQGSTNEALYKLYSAAATHRHLYHQAFPTPKSESTEQSEKKKM 相同性解析による交差性が予測される生物種 |
| Full Name | atlastin GTPase 1 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC010708 |
| Gene Symbol | ATL1 |
| Gene ID (NCBI) | 51062 |
| RRID | AB_3742494 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WXF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATL1 encodes atlastin-1, a GTPase that plays a crucial role in maintaining the structure and function of the endoplasmic reticulum. Mutations in ATL1 are one of the most common causes of hereditary spastic paraplegia (HSP) (PMID: 38851544). tlastin-1 is regulated by ubiquitination, a post-translational modification that can affect its activity and localization. For example, the E3 ubiquitin ligase SYVN1 has been shown to ubiquitinate atlastin-1, inhibiting its GTPase activity and thereby affecting ER morphology (PMID: 32916628).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATL1 antibody 31924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |