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Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of 3T3-L1 using 55190-1-AP (same clone as 55190-1-PBS)

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of A431 using 55190-1-AP (same clone as 55190-1-PBS)

    WB result of ATGL antibody (55190-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATGL transfected A431 cells. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis using 55190-1-AP (same clone as 55190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of rat heart using 55190-1-AP (same clone as 55190-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of mouse testis using 55190-1-AP (same clone as 55190-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of A431 using 55190-1-AP (same clone as 55190-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of human heart using 55190-1-AP (same clone as 55190-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of HeLa using 55190-1-AP (same clone as 55190-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATGL Polyclonal antibody (55190-1-AP)

    WB analysis of HeLa using 55190-1-AP (same clone as 55190-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55190-1-AP (ATGL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ATGL Polyclonal antibody (55190-1-AP)

    IP experiment of mouse testis using 55190-1-AP (same clone as 55190-1-PBS)

    IP result of anti-ATGL (IP:55190-1-AP, 4ug; Detection:55190-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 55190-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ATGL Polyclonal antibody, PBS Only

ATGL Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 55190-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Adipose triglyceride lipase, PNPLA2, Calcium-independent phospholipase A2-zeta, Desnutrin, EC:3.1.1.3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55190-1-PBS targets ATGL in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name patatin-like phospholipase domain containing 2
Calculated molecular weight 55 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberNM_020376
Gene Symbol ATGL
Gene ID (NCBI) 57104
RRIDAB_11182818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AD5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATGL, also named as PNPLA2, TTS2 and Desnutrin, catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. It also has acylglycerol transacylase activity. ATGL may act coordinately with LIPE/HLS within the lipolytic cascade. It regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. Defects in ATGL are the cause of neutral lipid storage disease with myopathy (NLSDM). This antibody is specific to ATGL.