Validation Data Gallery
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Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
Product Information
16924-1-AP targets ATG4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATG4D fusion protein Ag10307 相同性解析による交差性が予測される生物種 |
| Full Name | ATG4 autophagy related 4 homolog D (S. cerevisiae) |
| Calculated molecular weight | 474 aa, 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC068992 |
| Gene Symbol | ATG4D |
| Gene ID (NCBI) | 84971 |
| RRID | AB_2062024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86TL0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG4D, a member of autophagy-related protein 4 (ATG4) family, plays an interplay role between autophagy and apoptosis in cancers. There are two Atg4 family members-Atg4C and Atg4D-contain cryptic mitochondrial targeting sequences immediately downstream of their canonical(DEVD) caspase cleavage sites. Consequently, caspase-cleaved Atg4D (DN63 Atg4D) localizes to the mitochondrial matrix when expressed in mammalian cells, where it undergoes further processing to a ~42 kDa mitochondrial form.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG4D antibody 16924-1-AP | Download protocol |
| IF protocol for ATG4D antibody 16924-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell MST4 Phosphorylation of ATG4B Regulates Autophagic Activity, Tumorigenicity, and Radioresistance in Glioblastoma. | ||
Mol Cell Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine.
| ||
ACS Sens Air-Liquid Interface Microfluidic Monitoring Sensor Platform for Studying Autophagy Regulation after PM2.5 Exposure | ||
Cell Rep Subtractive CRISPR screen identifies the ATG16L1/vacuolar ATPase axis as required for non-canonical LC3 lipidation
| ||
Mol Ther Nucleic Acids lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation. | ||
Autophagy Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.
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