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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using ATG16L2 Polyclonal antibody (24322-1-AP)

    WB analysis of mouse spleen using 24322-1-AP (same clone as 24322-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ATG16L2 Polyclonal antibody (24322-1-AP)

    WB analysis of mouse testis using 24322-1-AP (same clone as 24322-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ATG16L2 Polyclonal antibody (24322-1-AP)

    IP experiment of mouse testis using 24322-1-AP (same clone as 24322-1-PBS)

    IP result of anti-ATG16L2 (IP:24322-1-AP, 3ug; Detection:24322-1-AP 1:700) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ATG16L2 Polyclonal antibody (24322-1-AP)

    IF Staining of MCF-7 using 24322-1-AP (same clone as 24322-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 24322-1-AP (ATG16L2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24322-1-PBS in a different storage buffer formulation.

ATG16L2 Polyclonal antibody, PBS Only

ATG16L2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 24322-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24322-1-PBS targets ATG16L2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19399

Product name: Recombinant human ATG16L2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-365 aa of BC137490

Sequence: HIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALRVKWQEEEEGLRLVCGEMAYQVVEKGAALGTLESELQQRQSRLAALEARVAQLREARAQQAQQVEEWRAQNAVQRAAYEALRAHVGLREAALRRLQEEARDLLERLVQRKARAAAERNLRNERRERAKQARVSQELKKAAKRTVSISEGPDTLGDGMRERRETLALAPEPEPLEKEACEKWKRPFRSASATSLTLSHCVDVVKGLLDFKKRRGHSIGGAPEQRYQIIPVCVAARLPTRAQDVLDAHLSEVNAVRFGPNSSLLATGGADRLIHLWNV

相同性解析による交差性が予測される生物種
Full Name ATG16 autophagy related 16-like 2 (S. cerevisiae)
Calculated molecular weight 619 aa, 69 kDa
Observed molecular weight 70 kDa, 30 kDa
GenBank accession numberBC137490
Gene Symbol ATG16L2
Gene ID (NCBI) 89849
RRIDAB_2879493
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NAA4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.

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