Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84935-6-RR targets ATG12 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ATG12 fusion protein Ag1791 相同性解析による交差性が予測される生物種 |
| Full Name | ATG12 autophagy related 12 homolog (S. cerevisiae) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC012266 |
| Gene Symbol | ATG12 |
| Gene ID (NCBI) | 9140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O94817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG12 antibody 84935-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |