Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells, NIH/3T3 cells, PC-3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30505-1-AP targets ATG12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32268 Product name: Recombinant human ATG12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 48-187 aa of BC012266 Sequence: MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG 相同性解析による交差性が予測される生物種 |
| Full Name | ATG12 autophagy related 12 homolog (S. cerevisiae) |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 20 kDa, 50-60 kDa |
| GenBank accession number | BC012266 |
| Gene Symbol | ATG12 |
| Gene ID (NCBI) | 9140 |
| RRID | AB_3086340 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATG12 antibody 30505-1-AP | Download protocol |
| IHC protocol for ATG12 antibody 30505-1-AP | Download protocol |
| WB protocol for ATG12 antibody 30505-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |