Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11264-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 11264-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HCT 116 using 11264-1-AP
HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11264-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 11264-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 11264-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11264-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11264-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11264-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 11264-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ATG12 antibody (11264-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11264-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

APG12, APG12 like, APG12L, APG12-like, Autophagy-related protein 12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, COLO 320 cells, NIH/3T3 cells, HeLa cells, HCT 116 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11264-1-AP targets ATG12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1791 Product name: Recombinant human ATG12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC012266 Sequence: MTSREHQVSLCNCVPLLRRLLCDAPWRKARPLHALSRYFRSRVSPSKMAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG 相同性解析による交差性が予測される生物種
Full Name	ATG12 autophagy related 12 homolog (S. cerevisiae)
Calculated molecular weight	15 kDa
Observed molecular weight	48-55 kDa
GenBank accession number	BC012266
Gene Symbol	ATG12
Gene ID (NCBI)	9140
RRID	AB_10807096
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94817
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

Protocols

Product Specific Protocols
IF protocol for ATG12 antibody 11264-1-AP	Download protocol
IHC protocol for ATG12 antibody 11264-1-AP	Download protocol
WB protocol for ATG12 antibody 11264-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy Authors - Lihao Jin View Article
rat	WB,IF	Front Cell Dev Biol Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat Authors - Huangrong Zhu View Article
human	WB	Biochem J Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway. Authors - Chunyang Du View Article
rat	WB	Biology (Basel) Autophagy Alters Bladder Angiogenesis and Improves Bladder Hyperactivity in the Pathogenesis of Ketamine-Induced Cystitis in a Rat Model. Authors - Jian-He Lu View Article
mouse,human	WB	Int J Biochem Cell Biol Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy. Authors - Chunyang Du View Article
human	WB	Eur J Pharmacol Latcripin-7A from Lentinula edodes C91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling. Authors - Syed Riaz Ud Din View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11264-1-AP

WB analysis using 11264-1-AP

WB analysis of HCT 116 using 11264-1-AP

WB analysis of COLO 320 using 11264-1-AP

WB analysis of NIH/3T3 using 11264-1-AP

IHC staining of human colon cancer using 11264-1-AP

IHC staining of human colon cancer using 11264-1-AP

IHC staining of human colon cancer using 11264-1-AP

IHC staining of human colon cancer using 11264-1-AP

IF Staining of PC-3 using 11264-1-AP

ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat

Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.

Autophagy Alters Bladder Angiogenesis and Improves Bladder Hyperactivity in the Pathogenesis of Ketamine-Induced Cystitis in a Rat Model.

Sphingosine kinase 1 protects renal tubular epithelial cells from renal fibrosis via induction of autophagy.

Latcripin-7A from Lentinula edodes C91-3 induces apoptosis, autophagy, and cell cycle arrest at G1 phase in human gastric cancer cells via inhibiting PI3K/Akt/mTOR signaling.