Validation Data Gallery
Tested Applications
| Positive WB detected in | human bladder tissue, UV treated HEK-293 |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 31 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
11122-1-AP targets ATG12 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1586 Product name: Recombinant human ATG12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC011033 Sequence: MTSREHQVSLCNCVPLLRRLLCDAPWRKARPLHALSRYFRSRVSPSKMAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG 相同性解析による交差性が予測される生物種 |
| Full Name | ATG12 autophagy related 12 homolog (S. cerevisiae) |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC011033 |
| Gene Symbol | ATG12 |
| Gene ID (NCBI) | 9140 |
| RRID | AB_2059368 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94817 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATG12 antibody 11122-1-AP | Download protocol |
| IP protocol for ATG12 antibody 11122-1-AP | Download protocol |
| WB protocol for ATG12 antibody 11122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell AMPK-Dependent Phosphorylation of GAPDH Triggers Sirt1 Activation and Is Necessary for Autophagy upon Glucose Starvation. | ||
Nat Commun Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling. | ||
Theranostics Down-regulated miR-23a Contributes to the Metastasis of Cutaneous Melanoma by Promoting Autophagy. | ||
Cancer Lett Let-7i-5p promotes a malignant phenotype in nasopharyngeal carcinoma via inhibiting tumor-suppressive autophagy. | ||
Cell Death Dis The lncRNA HULC functions as an oncogene by targeting ATG7 and ITGB1 in epithelial ovarian carcinoma. | ||
Am J Physiol Cell Physiol ALKBH5 promotes autophagy and progression by mediating m6A methylation of lncRNA UBOX5-AS1 in endometriosis |
