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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ATF7IP Polyclonal antibody (14699-1-AP)

    IHC staining of human breast cancer using 14699-1-AP (same clone as 14699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ATF7IP Polyclonal antibody (14699-1-AP)

    IHC staining of human breast cancer using 14699-1-AP (same clone as 14699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ATF7IP Polyclonal antibody (14699-1-AP)

    IHC staining of human ovary tumor using 14699-1-AP (same clone as 14699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATF7IP Polyclonal antibody (14699-1-AP)

    IHC staining of human stomach cancer using 14699-1-AP (same clone as 14699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ATF7IP Polyclonal antibody (14699-1-AP)

    IHC staining of human stomach cancer using 14699-1-AP (same clone as 14699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14699-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATF7IP Polyclonal antibody, PBS Only

ATF7IP Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 14699-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Synonyms

ATF-interacting protein, ATFa-associated modulator, ATF7-interacting protein, ATF7, ATF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14699-1-PBS targets ATF7IP in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6341

Product name: Recombinant human ATF7IP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 756-1106 aa of BC063855

Sequence: NTATVVATTQVPSGNPQPTISLQPLPVILHVPVAVSSQPQLLQSHPGTLVTNQPSGNVEFISVQSPPTVSGLTKNPVSLPSLPNPTKPNNVPSVPSPSIQRNPTASAAPLGTTLAVQAVPTAHSIVQATRTSLPTVGPSGLYSPSTNRGPIQMKIPISAFSTSSAAEQNSNTTPRIENQTNKTIDASVSKKAADSTSQCGKATGSDSSGVIDLTMDDEESGASQDPKKLNHTPVSTMSSSQPVSRPLQPIQPAPPLQPSGVPTSGPSQTTIHLLPTAPTTVNVTHRPVTQVTTRLPVPRAPANHQVVYTTLPAPPAQAPLRGTVMQAPAVRQVNPQNSKRFFLYMAPRYM

相同性解析による交差性が予測される生物種
Full Name activating transcription factor 7 interacting protein
Calculated molecular weight 137 kDa
GenBank accession numberBC063855
Gene Symbol ATF7IP
Gene ID (NCBI) 55729
RRIDAB_2878074
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6VMQ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATF7IP (Activating Transcription Factor 7 Interacting Protein), also known as MCAF1 (MBD1-containing chromatin-associated factor 1), is a multifunctional scaffold protein that plays a pivotal role in epigenetic regulation and transcriptional control. Functionally, ATF7IP acts as an adaptor that recruits the histone methyltransferase SETDB1 to specific genomic loci, facilitating the deposition of the heterochromatin mark H3K9me3 and silencing target genes, including retrotransposons such as LINE1. This activity is essential for maintaining genomic stability by suppressing transposable element mobilization and preventing DNA damage.

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