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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATF1 Recombinant monoclonal antibody (87421-1-RR)

    WB analysis using 87421-1-RR (same clone as 87421-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87421-1-RR (ATF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ATF1 Recombinant monoclonal antibody (87421-1-RR)

    WB analysis of A549 using 87421-1-RR (same clone as 87421-1-PBS)

    WB result of ATF1 antibody (87421-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF1 transfected A549 cells. This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATF1 Recombinant monoclonal antibody (87421-1-RR)

    IF Staining of HeLa using 87421-1-RR (same clone as 87421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ATF1 Recombinant monoclonal antibody (87421-1-RR)

    IF Staining of NIH/3T3 using 87421-1-RR (same clone as 87421-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ATF1 antibody (87421-1-RR, Clone: 252816B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87421-1-PBS in a different storage buffer formulation.

ATF1 Recombinant monoclonal antibody, PBS Only

ATF1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87421-1-PBS
Clone No.252816B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

cAMP-dependent transcription factor ATF-1, Cyclic AMP-dependent transcription factor ATF-1, TREB36

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87421-1-PBS targets ATF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2548

Product name: Recombinant human ATF1 protein

Source: e coli.-derived, T-GST

Tag: GST

Domain: 1-271 aa of BC029619

Sequence: MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSV

相同性解析による交差性が予測される生物種
Full Name activating transcription factor 1
Calculated molecular weight 271 aa, 29 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC029619
Gene Symbol ATF1
Gene ID (NCBI) 466
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP18846
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

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