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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATAD2 Polyclonal antibody (23894-1-AP)

    WB analysis of HeLa using 23894-1-AP (same clone as 23894-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23894-1-AP (ATAD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23894-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATAD2 Polyclonal antibody (23894-1-AP)

    IP experiment of HeLa using 23894-1-AP (same clone as 23894-1-PBS)

    IP result of anti-ATAD2 (IP:23894-1-AP, 4ug; Detection:23894-1-AP 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23894-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATAD2 Polyclonal antibody (23894-1-AP)

    IF Staining of HeLa using 23894-1-AP (same clone as 23894-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23894-1-AP (ATAD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23894-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATAD2 Polyclonal antibody, PBS Only

ATAD2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 23894-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23894-1-PBS targets ATAD2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20947

Product name: Recombinant human ATAD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of BC113656

Sequence: MVVLRSSLELHNHSAASATGSLDLSSDFLSLEHIGRRRLRSAGAAQKKPAATTAKAGDGSSVKEVETYHRTRALRSLRKDAQNSSDSSFEKNVEITEQLANGRHFTRQLARQQADKKKEEHREDKVIPVTRSLRARNIVQSTEHLHEDNG

相同性解析による交差性が予測される生物種
Full Name ATPase family, AAA domain containing 2
Calculated molecular weight 1390 aa, 159 kDa
Observed molecular weight 159 kDa
GenBank accession numberBC113656
Gene Symbol ATAD2
Gene ID (NCBI) 29028
RRIDAB_2879352
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PL18
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATAD2 is one of them encoding a conserved factor harbouring an AAA type ATPase domain and a bromodomain. ATAD2 controls chromatin dynamics, genome transcriptional activities and apoptotic cell response. This protein has 2 isoforms produced by alternative splicing.

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