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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASXL2 Polyclonal antibody (27229-1-AP)

    WB analysis using 27229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27229-1-AP (ASXL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ASXL2 Polyclonal antibody

ASXL2 Polyclonal Antibody for WB, ELISA
Cat No. 27229-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293T cells, Jurkat cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27229-1-AP targets ASXL2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASXL2 fusion protein Ag24402 相同性解析による交差性が予測される生物種
Full Name additional sex combs like 2 (Drosophila)
Calculated molecular weight 154 kDa
Observed molecular weight154-230 kDa
GenBank accession numberBC042999
Gene Symbol ASXL2
Gene ID (NCBI) 55252
RRIDAB_3669589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ76L83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASXL2 is an epigenetic regulator associated with various tumors including colorectal cancer, breast cancer, and myeloid leukemia. ASXL1, ASXL2 and ASXL3 are human homologs of Drosophila Asx (Additional sex combs) and function as epigenetic regulators through recruitment of Polycomb group repressor complexes (PRC) and Trithorax group activator complexes. ASXL proteins can interact with BAP1, NCOA1, EZH2, WTIP and nuclear receptors, which suggests diverse functions of ASXL family members in epigenetic and transcriptional regulation. (PMID: 30093396, PMID: 34692512)

Protocols

Product Specific Protocols
WB protocol for ASXL2 antibody 27229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols