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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ASPN Polyclonal antibody (27602-1-AP)

    WB analysis of HepG2 using 27602-1-AP (same clone as 27602-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ASPN Polyclonal antibody (27602-1-AP)

    WB analysis using 27602-1-AP (same clone as 27602-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27602-1-AP (ASPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ASPN Polyclonal antibody (27602-1-AP)

    IHC staining of human stomach cancer using 27602-1-AP (same clone as 27602-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27602-1-AP (ASPN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27602-1-PBS in a different storage buffer formulation.

ASPN Polyclonal antibody, PBS Only

ASPN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27602-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

SLRR1C, PLAP-1, PLAP1, PLAP 1, Periodontal ligament-associated protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27602-1-PBS targets ASPN in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26380

Product name: Recombinant human ASPN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 53-330 aa of BC063114

Sequence: DDEEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIAR

相同性解析による交差性が予測される生物種
Full Name asporin
Calculated molecular weight 384 aa, 44 kDa
Observed molecular weight43 kDa
GenBank accession numberBC063114
Gene Symbol ASPN
Gene ID (NCBI) 54829
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXN1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ASPN, also known as periodontal ligament-associated protein1 (PLAP1), is an extracellular matrix (ECM) protein. The calculated molecular weight of ASPN is 43 kDa. It belongs to the small leucine-rich proteoglycans (SLRPs) family and contains a unique aspartate-rich N terminus that distinguishes it from other SLRPs family members. It was firstly identified in human cartilage and it was associated with the pathogenesis of bone and joint diseases, including osteoarthritis, intervertebral disc degeneration and periodontal ligament mineralization (PMID:11152692; 26089687).

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