Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 26223-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26223-1-AP (ASPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human gliomas using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 26223-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 26223-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26223-1-AP (ASPM antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with alpha tubulin (66031-1-Ig, red).

Featured Product
KD/KO Validated

ASPM Polyclonal antibody

ASPM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26223-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ASP, Asp homolog, ASPM, Calmbp1, MCPH5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (25)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, K-562 cells
Positive IHC detected in	human prostate cancer tissue, human gliomas tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 15 publications below
IHC	See 12 publications below
IF	See 9 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

26223-1-AP targets ASPM in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24166 Product name: Recombinant human ASPM protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-95 aa of BC034607 Sequence: MSLRAYTARCRLNRLRRAACRLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLEDNSDVT 相同性解析による交差性が予測される生物種
Full Name	asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
Calculated molecular weight	410 kDa or 218 kDa
Observed molecular weight	218 kDa
GenBank accession number	BC034607
Gene Symbol	ASPM
Gene ID (NCBI)	259266
RRID	AB_2880431
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IZT6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for ASPM antibody 26223-1-AP	Download protocol
IHC protocol for ASPM antibody 26223-1-AP	Download protocol
WB protocol for ASPM antibody 26223-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Science The mechanism of acentrosomal spindle assembly in human oocytes Authors - Tianyu Wu View Article
human	WB,IF	Exploration (Beijing) ASPM Induces Radiotherapy Resistance by Disrupting Microtubule Stability Leading to Chromosome Malsegregation in Non-Small Cell Lung Cancer. Authors - Tao Zhong KD Validated View Article
human	WB,IF	Cancer Lett ZBTB20 promotes malignancy and stemness in lung squamous cell carcinoma through the transcriptional repression of KCTD1 and activation of Hedgehog signaling. Authors - Hai Wang View Article
human	WB,IHC,IF	Genome Biol ASPM mediates nuclear entrapment of FOXM1 via liquid-liquid phase separation to promote progression of hepatocarcinoma Authors - Xunliang Jiang KD Validated View Article
mouse	IF	EMBO J Binary organization of epidermal basal domains highlights robustness to environmental exposure Authors - Sangeeta Ghuwalewala View Article
	WB	Cell Rep KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C. Authors - Jiahang Miao View Article

Validation Data Gallery

WB analysis using 26223-1-AP

IHC staining of human prostate cancer using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human gliomas using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human ovary tumor using 26223-1-AP

IHC staining of human prostate cancer using 26223-1-AP

IHC staining of human prostate cancer using 26223-1-AP

IF Staining of HeLa using 26223-1-AP

ASPM Polyclonal antibody

ASPM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The mechanism of acentrosomal spindle assembly in human oocytes

ASPM Induces Radiotherapy Resistance by Disrupting Microtubule Stability Leading to Chromosome Malsegregation in Non-Small Cell Lung Cancer.

ZBTB20 promotes malignancy and stemness in lung squamous cell carcinoma through the transcriptional repression of KCTD1 and activation of Hedgehog signaling.

ASPM mediates nuclear entrapment of FOXM1 via liquid-liquid phase separation to promote progression of hepatocarcinoma

Binary organization of epidermal basal domains highlights robustness to environmental exposure

KIF18A promotes chromosome congression in cooperation with CENP-E downstream of CENP-C.

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