Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
13244-1-AP targets ASPA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4029 Product name: Recombinant human ASPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC029128 Sequence: MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH 相同性解析による交差性が予測される生物種 |
| Full Name | aspartoacylase (Canavan disease) |
| Calculated molecular weight | 313 aa, 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC029128 |
| Gene Symbol | ASPA |
| Gene ID (NCBI) | 443 |
| RRID | AB_2274358 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P45381 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ASPA(Aspartoacylase) also called aminoacylase-2, is an enzyme that hydrolyzes N-acetyl-L-aspartic acid (NAA) to aspartate and acetate.Human ASPA encodes a deduced 313-amino acid protein with a molecular mass of 36 KDa. It shares 92% sequence identity with the bovine protein and contains 1 potential N-glycosylation site and 5 phosphorylation sites.Defects in ASPA are the cause of Canavan disease (CAND).This protein can exsit as a homodimer(18293939).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ASPA antibody 13244-1-AP | Download protocol |
| WB protocol for ASPA antibody 13244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mil Med Res Aspartoacylase suppresses prostate cancer progression by blocking LYN activation | ||
Elife Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. | ||
Glia Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome | ||
J Neurosci Suppressing N-Acetyl-l-Aspartate Synthesis Prevents Loss of Neurons in a Murine Model of Canavan Leukodystrophy.
| ||
Front Pharmacol Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress. | ||
J Med Virol HPV-CCDC106 integration promotes cervical cancer progression by facilitating the high expression of CCDC106 after HPV E6 splicing. |
