Validation Data Gallery

Filter: AllWBIHCIF

WB analysis of HEK-293 using 67072-1-Ig (same clone as 67072-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 67072-1-Ig (same clone as 67072-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 67072-1-Ig (same clone as 67072-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 67072-1-Ig (same clone as 67072-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 67072-1-Ig (same clone as 67072-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 67072-1-Ig (same clone as 67072-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 67072-1-Ig (same clone as 67072-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 67072-1-Ig (same clone as 67072-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67072-1-Ig (same clone as 67072-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67072-1-Ig (same clone as 67072-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.
IF Staining of RAW 264.7 using 67072-1-Ig (same clone as 67072-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67072-1-PBS in a different storage buffer formulation.

ASK1 Monoclonal antibody, PBS Only

ASK1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67072-1-PBS

Clone No.1C3A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67072-1-PBS targets ASK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5818 Product name: Recombinant human ASK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 681-936 aa of BC054503 Sequence: GDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDE 相同性解析による交差性が予測される生物種
Full Name	mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight	155 kDa
Observed molecular weight	110 kDa
GenBank accession number	BC054503
Gene Symbol	ASK1
Gene ID (NCBI)	4217
RRID	AB_2882381
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q99683
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 67072-1-Ig (same clone as 67072-1-PBS)

WB analysis of HSC-T6 using 67072-1-Ig (same clone as 67072-1-PBS)

WB analysis of NIH/3T3 using 67072-1-Ig (same clone as 67072-1-PBS)

WB analysis of MCF-7 using 67072-1-Ig (same clone as 67072-1-PBS)

WB analysis of Jurkat using 67072-1-Ig (same clone as 67072-1-PBS)

WB analysis of HeLa using 67072-1-Ig (same clone as 67072-1-PBS)

IHC staining of human pancreas cancer using 67072-1-Ig (same clone as 67072-1-PBS)