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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G1H3

Cat no : 67859-1-Ig

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

Validation Data Gallery

Applications:
  • WB analysis using 67859-1-Ig

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67859-1-Ig

    WB analysis using 67859-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67859-1-Ig (ASH2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67859-1-Ig

    IHC staining of human colon cancer using 67859-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67859-1-Ig (ASH2L antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASH2L antibody included
  • FC experiment of HeLa using 67859-1-Ig

    FC experiment of HeLa using 67859-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ASH2L (67859-1-Ig, Clone:1G1H3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67859-1-Ig targets ASH2L in WB, FC, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ASH2L fusion protein Ag3008 相同性解析による交差性が予測される生物種
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC015936
Gene symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2918617
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASH2L antibody 67859-1-IgDownload protocol
IHC protocol for ASH2L antibody 67859-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols