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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ASH2L Polyclonal antibody

ASH2L Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12331-1-AP

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

Validation Data Gallery

Applications:
  • WB analysis of COS-7 using 12331-1-AP

    WB analysis of COS-7 using 12331-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12331-1-AP

    WB analysis of COLO 320 using 12331-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12331-1-AP

    WB analysis of COLO 320 using 12331-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12331-1-AP

    WB analysis of COLO 320 using 12331-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12331-1-AP

    WB analysis of COLO 320 using 12331-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12331-1-AP (ASH2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 12331-1-AP

    IP experiment of COLO 320 using 12331-1-AP

    IP result of anti-ASH2L (IP:12331-1-AP, 4ug; Detection:12331-1-AP 1:500) with COLO 320 cells lysate 2000ug.

  • IHC staining of human gliomas using 12331-1-AP

    IHC staining of human gliomas using 12331-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASH2L antibody included
  • IHC staining of human gliomas using 12331-1-AP

    IHC staining of human gliomas using 12331-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12331-1-AP (ASH2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASH2L antibody included

Tested Applications

Positive WB detected inCOS-7 cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ASH2L Ready-To-Use IHC Kit. ASH2L primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12331-1-AP targets ASH2L in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASH2L fusion protein Ag3008 相同性解析による交差性が予測される生物種
Full Name ash2 (absent, small, or homeotic)-like (Drosophila)
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC015936
Gene symbol ASH2L
Gene ID (NCBI) 9070
RRIDAB_2059837
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASH2L antibody 12331-1-APDownload protocol
IHC protocol for ASH2L antibody 12331-1-APDownload protocol
IP protocol for ASH2L antibody 12331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation.

Authors - Esteban A Orellana
View Article
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
View Article
humanWB,IHC,IF,IP,ChIP

Cancer Sci

ASH2L is involved in promotion of endometrial cancer progression via upregulation of PAX2 transcription.

Authors - Kai Zeng
  • KD Validated
View Article
mouseWB,IP

Mol Neurobiol

WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.

Authors - Minhan Ka
View Article