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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASGR1 Polyclonal antibody (31338-1-AP)

    WB analysis using 31338-1-AP (same clone as 31338-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31338-1-AP (ASGR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ASGR1 Polyclonal antibody (31338-1-AP)

    IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using ASGR1 Polyclonal antibody (31338-1-AP)

    IHC staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ASGR1 Polyclonal antibody (31338-1-AP)

    IHC staining of mouse liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ASGR1 Polyclonal antibody (31338-1-AP)

    IHC staining of mouse liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31338-1-AP (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using ASGR1 Polyclonal antibody (31338-1-AP)

    IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using ASGR1 Polyclonal antibody (31338-1-AP)

    IF Staining of rat liver using 31338-1-AP (same clone as 31338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (31338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31338-1-PBS in a different storage buffer formulation.

ASGR1 Polyclonal antibody, PBS Only

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31338-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31338-1-PBS targets ASGR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name asialoglycoprotein receptor 1
Calculated molecular weight33 kDa
Observed molecular weight38-40 kDa
GenBank accession numberNM_001671.4
Gene Symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_3669951
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07306-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

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