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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ASF1A Monoclonal antibody (66884-1-Ig)

    WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using ASF1A Monoclonal antibody (66884-1-Ig)

    WB analysis of HSC-T6 using 66884-1-Ig (same clone as 66884-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASF1A Monoclonal antibody (66884-1-Ig)

    IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF1A antibody (66884-1-Ig, Clone: 2D4A6 ) at dilution of 1:500 and sms3AF647-1, CL488-Phalloidin (green). This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51470-1

    Cytometric bead array sample test of MP51470-1

    Cytometric bead array sample test of MP51470-1, ASF1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66884-2-PBS. Detection antibody: 66884-1-PBS.

  • Cytometric bead array standard curve of MP51470-1

    Cytometric bead array standard curve of MP51470-1

    Cytometric bead array standard curve of MP51470-1, ASF1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66884-2-PBS. Detection antibody: 66884-1-PBS. Standard:Ag1233. Range: 0.781-100 ng/mL.

ASF1A Monoclonal antibody, PBS Only (Detector)

ASF1A Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66884-1-PBS
Clone No.2D4A6

Host / Isotype

Mouse / IgG3

Reactivity

human, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

2D4A6, Anti-silencing function protein 1 homolog A, CCG1 interacting factor A, CCG1-interacting factor A, CGI 98

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66884-1-PBS targets ASF1A as part of a matched antibody pair:

MP51470-1: 66884-2-PBS capture and 66884-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1233

Product name: Recombinant human ASF1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC010878

Sequence: MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

相同性解析による交差性が予測される生物種
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight 21-23 kDa
GenBank accession numberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2882216
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y294
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.