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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    WB analysis using 67494-1-Ig (same clone as 67494-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    WB analysis of human placenta using 67494-1-Ig (same clone as 67494-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    WB analysis of HL-60 using 67494-1-Ig (same clone as 67494-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    IHC staining of human colon cancer using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    IF Staining of HeLa using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    IF Staining of MCF-7 using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using ASC/TMS1 Monoclonal antibody (67494-1-Ig)

    IF Staining of THP-1 using 67494-1-Ig (same clone as 67494-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ASC/TMS1 antibody (67494-1-Ig, Clone: 2G12E6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

ASC/TMS1 Monoclonal antibody, PBS Only

ASC/TMS1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67494-1-PBS
Clone No.2G12E6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PYCARD, 2G12E6, Apoptosis-associated speck-like protein containing a CARD, ASC, CARD5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67494-1-PBS targets ASC/TMS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28424

Product name: Recombinant human TMS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-135 aa of BC004470

Sequence: MGRARDAILDALENLTAEELKKFKLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS

相同性解析による交差性が予測される生物種
Full Name PYD and CARD domain containing
Calculated molecular weight 25 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC004470
Gene Symbol ASC/TMS1
Gene ID (NCBI) 29108
RRIDAB_2882718
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9ULZ3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

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