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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using ASB8 Polyclonal antibody (11735-1-AP)

    WB analysis of human heart using 11735-1-AP (same clone as 11735-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11735-1-AP (ASB8 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11735-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using ASB8 Polyclonal antibody (11735-1-AP)

    WB analysis of human heart using 11735-1-AP (same clone as 11735-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11735-1-AP (ASB8 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ASB8 Polyclonal antibody (11735-1-AP)

    IHC staining of human colon cancer using 11735-1-AP (same clone as 11735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11735-1-AP (ASB8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11735-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASB8 Polyclonal antibody (11735-1-AP)

    IF Staining of HepG2 using 11735-1-AP (same clone as 11735-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11735-1-AP (ASB8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11735-1-PBS in a different storage buffer formulation.

ASB8 Polyclonal antibody, PBS Only

ASB8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11735-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11735-1-PBS targets ASB8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2361

Product name: Recombinant human ASB8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-288 aa of BC001321

Sequence: MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGGADVNCTHGTLKPLHCACMVSDADCVELLLEKGAEVNALDGYNRTALHYAAEKDEACVEVLLEYGANPNALDGNRDTPLHWAAFKNNAECVRALLESGASVNALDYNNDTPLSWAAMKGNLESVSILLDYGAEVRVINLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHFELRKNGTMPREVARDPQLCEKLTVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPLPASLKEYLLLLE

相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and SOCS box-containing 8
Calculated molecular weight 288 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001321
Gene Symbol ASB8
Gene ID (NCBI) 140461
RRIDAB_2227489
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H765
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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