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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ASB6 Polyclonal antibody

ASB6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21449-1-AP

Synonyms

ASB 6, ASB6

Validation Data Gallery

Applications:
  • WB analysis of L02 using 21449-1-AP

    WB analysis of L02 using 21449-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21449-1-AP (ASB6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 21449-1-AP

    WB analysis of L02 using 21449-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21449-1-AP (ASB6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 21449-1-AP

    WB analysis of HEK-293 using 21449-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21449-1-AP (ASB6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inL02 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21449-1-AP is ASB6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASB6 fusion protein Ag13863 相同性解析による交差性が予測される生物種
Full Name ankyrin repeat and SOCS box-containing 6
Calculated molecular weight 421 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001719
Gene symbol ASB6
Gene ID (NCBI) 140459
RRIDAB_2878860
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASB6 antibody 21449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

CUL5-ASB6 Complex Promotes p62/SQSTM1 Ubiquitination and Degradation to Regulate Cell Proliferation and Autophagy.

Authors - Liyan Gong
View Article