Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, mouse testis tissue, rat skeletal musle tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33262-1-AP targets ASB12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36991 Product name: Recombinant human ASB12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 12-161 aa of BC069436 Sequence: LLQPDKEEEDTDTEEKQALNQAVYDNDSYTLDQLLRQERYKRFINSRSGWGVPGTPLRLAASYGHLSCLQVLLAHGADVDSLDVKAQTPLFTAVSHGHLDCVRVLLEAGASPGGSIYNNCSPVLTAARDGAVAILQELLDHGAEANVKAK 相同性解析による交差性が予測される生物種 |
| Full Name | ankyrin repeat and SOCS box-containing 12 |
| Calculated molecular weight | 309 aa, 34 kDa/318 aa, 35 kDa |
| Observed molecular weight | 34,39 kDa |
| GenBank accession number | BC069436 |
| Gene Symbol | ASB12 |
| Gene ID (NCBI) | 142689 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8WXK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ankyrin repeat and SOCS box (ASB) family is composed of 18 proteins from ASB1 to ASB18 and belongs to the suppressor of cytokine signaling (SOCS) box protein superfamily. ASB family proteins interact with Cul5-Rbx2 to form E3 Ub ligases and play significant roles via a ubiquitination-mediated pathway (PMID: 16325183). The calculated molecular weight of ASB12 in mice or rat was 34 kda. ASB12 may be ubiquitinated, resulting in an increase in the observed molecular quantity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASB12 antibody 33262-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |