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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using ARX Polyclonal antibody (20739-1-AP)

    WB analysis of NIH/3T3 using 20739-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ARX Polyclonal antibody (20739-1-AP)

    WB analysis of SH-SY5Y using 20739-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARX Polyclonal antibody (20739-1-AP)

    WB analysis of NIH/3T3 using 20739-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARX Polyclonal antibody (20739-1-AP)

    WB analysis of NIH/3T3 using 20739-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ARX Polyclonal antibody (20739-1-AP)

    WB analysis of SH-SY5Y using 20739-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20739-1-AP (ARX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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ARX Polyclonal antibody

ARX Polyclonal Antibody for WB, ELISA
Cat No. 20739-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

aristaless related homeobox, ARX, Homeobox protein ARX, ISSX, MRX29, MRX32, MRX33, MRX36, MRX38, MRX43, MRX54, MRX76, MRX87, MRXS1, PRTS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20739-1-AP targets ARX in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name aristaless related homeobox
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_139058
Gene Symbol ARX
Gene ID (NCBI) 170302
RRIDAB_2878729
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QS3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARX belongs to the paired homeobox family and Bicoid subfamily. It is a transcription factor which required for normal brain development. ARX may be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate. Defects in ARX are the cause of lissencephaly X-linked type 2 (LISX2), epileptic encephalopathy early infantile type 1 (EIEE1), Partington syndrome (PRTS), mental retardation X-linked ARX-related (MRXARX), and agenesis of the corpus callosum with abnormal genitalia (ACCAG). The antibody is specific to ARX. This is a rabbit polyclonal antibody raised against a peptide mapping within human ARX.

Protocols

Product Specific Protocols
WB protocol for ARX antibody 20739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols