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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ART5 Polyclonal antibody

ART5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11354-1-AP

Synonyms

ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5

Validation Data Gallery

Applications:
  • WB analysis of mouse testis using 11354-1-AP

    WB analysis of mouse testis using 11354-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11354-1-AP (ART5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung cancer using 11354-1-AP

    IHC staining of human lung cancer using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 11354-1-AP

    IHC staining of human lung cancer using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11354-1-AP targets ART5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ART5 fusion protein Ag1786 相同性解析による交差性が予測される生物種
Full Name ADP-ribosyltransferase 5
Calculated molecular weight 292 aa, 32 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014577
Gene symbol ART5
Gene ID (NCBI) 116969
RRIDAB_10734329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ART5 (Ecto-ADP-ribosyltransferase 5) belongs to a class of enzymes that catalyze the transfer of an ADP-ribose group from NAD to arginine residues on target proteins like G proteins. ART5 is a secreted protein. This antibody detects the 43 kDa ART5 protein in mouse liver and testis.

Protocols

Product Specific Protocols
WB protocol for ART5 antibody 11354-1-APDownload protocol
IHC protocol for ART5 antibody 11354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols