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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ART3 Polyclonal antibody (15930-1-AP)

    WB analysis using 15930-1-AP (same clone as 15930-1-PBS)

    Various tissues lysates were subjected to SDS PAGE followed by western blot with 15930-1-AP (ART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using ART3 Polyclonal antibody (15930-1-AP)

    WB analysis of rat testis using 15930-1-AP (same clone as 15930-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 15930-1-AP (ART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ART3 Polyclonal antibody (15930-1-AP)

    IHC staining of mouse testis using 15930-1-AP (same clone as 15930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15930-1-AP (ART3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using ART3 Polyclonal antibody (15930-1-AP)

    IHC staining of rat testis using 15930-1-AP (same clone as 15930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15930-1-AP (ART3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15930-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ART3 Polyclonal antibody, PBS Only

ART3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15930-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ADP ribosyltransferase 3, ADP-ribosyltransferase C2 and C3 toxin-like 3, ARTC3, EC:2.4.2.31, Ecto ADP ribosyltransferase 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15930-1-PBS targets ART3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8775

Product name: Recombinant human ART3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-364 aa of BC008397

Sequence: EVLDMADNAFDDEYLKCTDRMEIKYVPQLLKEEKASHQQLDTVWENAKAKWAARKTQIFLPMNFKDNHGIALMAYISEAQEQTPFYHLFSEAVKMAGQSREDYIYGFQFKAFHFYLTRALQLLRKPCEASSKTVVYRTSQGTSFTFGGLNQARFGHFTLAYSAKPQAANDQLTVLSIYTCLGVDIENFLDKESERITLIPLNEVFQVSQEGAGNNLILQSINKTCSHYECAFLGGLKTENCIENLEYFQPIYVYNPGEKNQKLEDHSEKNWKLEDHGEKNQKLEDHGVKILEPTQIPGMKIPEPFPLPEDKSQGNINNPTPGPVPVPGPKSHPSASLG

相同性解析による交差性が予測される生物種
Full Name ADP-ribosyltransferase 3
Calculated molecular weight 389 aa, 44 kDa
Observed molecular weight38-47 kDa
GenBank accession numberBC008397
Gene Symbol ART3
Gene ID (NCBI) 419
RRIDAB_2060870
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13508
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ART3 is a GPI-anchored mono-ADP-ribosyltransferase enriched on the sperm cell surface, where it participates in spermatid maturation and membrane remodeling. Localized to lipid rafts, ART3 likely modulates cell-cell signaling and extracellular protein modification. Its upregulation in multiple cancers suggests additional roles in migration, invasion, and tumor aggressiveness. Despite incomplete characterization of its enzymatic activity, ART3 remains a key regulator at the interface of reproductive biology, membrane signaling, and pathology.

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