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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ARSB Polyclonal antibody (13227-1-AP)

    WB analysis of HepG2 using 13227-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ARSB Polyclonal antibody (13227-1-AP)

    WB analysis of HepG2 using 13227-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARSB Polyclonal antibody (13227-1-AP)

    IHC staining of human placenta using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ARSB Polyclonal antibody (13227-1-AP)

    IHC staining of human placenta using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARSB Polyclonal antibody (13227-1-AP)

    IHC staining of human breast cancer using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARSB Polyclonal antibody (13227-1-AP)

    IHC staining of human breast cancer using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ARSB Polyclonal antibody (13227-1-AP)

    IHC staining of human heart using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ARSB Polyclonal antibody (13227-1-AP)

    IHC staining of human heart using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

ARSB Polyclonal antibody

ARSB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13227-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

ARSB, arylsulfatase B, ASB, G4S, MPS6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13227-1-AP targets ARSB in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARSB fusion protein Ag4083 相同性解析による交差性が予測される生物種
Full Name arylsulfatase B
Calculated molecular weight 413 aa, 46 kDa
Observed molecular weight60 kDa, 44 kDa
GenBank accession numberBC029051
Gene Symbol ARSB
Gene ID (NCBI) 411
RRIDAB_2227579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARSB antibody 13227-1-APDownload protocol
IHC protocol for ARSB antibody 13227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Oxid Med Cell Longev

miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling.

Authors - Que Wang
  • KD Validated
View Article
mouseWB

Elife

Chondroitin sulfate proteoglycan 4,6 sulfation regulates sympathetic nerve regeneration after myocardial infarction.

Authors - Matthew R Blake
View Article
mouseWB,IF

Biochem Biophys Rep

Generation of a novel disease model mouse for mucopolysaccharidosis type VI via c. 252T>C human ARSB mutation knock-in

Authors - Kosuke Hosoba
View Article