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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ARSB Polyclonal antibody

ARSB Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13227-1-AP

Synonyms

ARSB, arylsulfatase B, ASB, G4S, MPS6

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 13227-1-AP

    WB analysis of HepG2 using 13227-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 13227-1-AP

    WB analysis of HepG2 using 13227-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13227-1-AP (ARSB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13227-1-AP

    IHC staining of human placenta using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 13227-1-AP

    IHC staining of human placenta using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 13227-1-AP

    IHC staining of human breast cancer using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 13227-1-AP

    IHC staining of human breast cancer using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 13227-1-AP

    IHC staining of human heart using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 13227-1-AP

    IHC staining of human heart using 13227-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13227-1-AP (ARSB Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13227-1-AP targets ARSB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARSB fusion protein Ag4083 相同性解析による交差性が予測される生物種
Full Name arylsulfatase B
Calculated molecular weight 413 aa, 46 kDa
Observed molecular weight60 kDa, 44 kDa
GenBank accession numberBC029051
Gene symbol ARSB
Gene ID (NCBI) 411
RRIDAB_2227579
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARSB antibody 13227-1-APDownload protocol
IHC protocol for ARSB antibody 13227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Oxid Med Cell Longev

miR-154-5p Functions as an Important Regulator of Angiotensin II-Mediated Heart Remodeling.

Authors - Que Wang
  • KD Validated
View Article
mouseWB

Elife

Chondroitin sulfate proteoglycan 4,6 sulfation regulates sympathetic nerve regeneration after myocardial infarction.

Authors - Matthew R Blake
View Article
mouseWB,IF

Biochem Biophys Rep

Generation of a novel disease model mouse for mucopolysaccharidosis type VI via c. 252T>C human ARSB mutation knock-in

Authors - Kosuke Hosoba
View Article