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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARPP-21 Polyclonal antibody

ARPP-21 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11829-1-AP

Synonyms

ARPP 21, ARPP21, ARPP-21, RCS, TARPP

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 11829-1-AP

    WB analysis of mouse brain using 11829-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11829-1-AP (ARPP-21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11829-1-AP

    IHC staining of mouse brain using 11829-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11829-1-AP (ARPP-21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11829-1-AP

    IHC staining of mouse brain using 11829-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11829-1-AP (ARPP-21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cerebellum using 11829-1-AP

    IHC staining of human cerebellum using 11829-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11829-1-AP (ARPP-21 Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human cerebellum using 11829-1-AP

    IHC staining of human cerebellum using 11829-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 11829-1-AP (ARPP-21 Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

11829-1-AP targets ARPP-21 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPP-21 fusion protein Ag2399 相同性解析による交差性が予測される生物種
Full Name cyclic AMP-regulated phosphoprotein, 21 kD
Calculated molecular weight 89 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC017805
Gene symbol ARPP-21
Gene ID (NCBI) 10777
RRIDAB_2877798
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARPP-21 antibody 11829-1-APDownload protocol
IHC protocol for ARPP-21 antibody 11829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.

Authors - Ye-Ya Tan
View Article
mouseWB

Nat Commun

The thymocyte-specific RNA-binding protein Arpp21 provides TCR repertoire diversity by binding to the 3'-UTR and promoting Rag1 mRNA expression

Authors - Meng Xu
View Article