Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 15058-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 15058-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15058-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15058-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15058-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 15058-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15058-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 15058-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15058-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15058-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15058-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15058-1-AP
IP result of anti-ARPC2 (IP:15058-1-AP, 4ug; Detection:15058-1-AP 1:6000) with HeLa cells lysate 1200 ug.
IHC staining of human breast cancer using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15058-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARPC2 antibody (15058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 15058-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (15058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

ARPC2 Polyclonal antibody

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15058-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Actin-related protein 2/3 complex subunit 2, ARC34, p34 Arc, p34-ARC

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-1080 cells, HeLa cells, mouse skeletal muscle tissue, PC-3 cells, 3T3-L1 cells, NIH/3T3 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human colon tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

15058-1-AP targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7041 Product name: Recombinant human ARPC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC000590 Sequence: MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR 相同性解析による交差性が予測される生物種
Full Name	actin related protein 2/3 complex, subunit 2, 34kDa
Calculated molecular weight	34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC000590
Gene Symbol	ARPC2
Gene ID (NCBI)	10109
RRID	AB_2059787
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15144
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for ARPC2 antibody 15058-1-AP	Download protocol
IF protocol for ARPC2 antibody 15058-1-AP	Download protocol
IHC protocol for ARPC2 antibody 15058-1-AP	Download protocol
IP protocol for ARPC2 antibody 15058-1-AP	Download protocol
WB protocol for ARPC2 antibody 15058-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Dev Cell Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages Authors - Xinyi Huang View Article
mouse	WB	Dev Cell Aberrant cortex contractions impact mammalian oocyte quality Authors - Elvira Nikalayevich View Article
	WB	Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein Authors - Megan L Norris View Article
human	WB	J Cell Biol α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3. Authors - Nikki R Paul KD Validated View Article
human	IP	EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. Authors - Kailiang Zhao View Article
human	WB	J Neurosci The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse. Authors - Avanti Gokhale View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15058-1-AP

WB analysis of MCF-7 using 15058-1-AP

WB analysis of HeLa using 15058-1-AP

WB analysis of HeLa using 15058-1-AP

WB analysis of mouse skeletal muscle using 15058-1-AP

WB analysis of mouse skeletal muscle using 15058-1-AP

WB analysis of PC-3 using 15058-1-AP

WB analysis of 3T3-L1 using 15058-1-AP

WB analysis of PC-3 using 15058-1-AP

WB analysis of HeLa using 15058-1-AP

WB analysis of NIH/3T3 using 15058-1-AP

IP experiment of HeLa using 15058-1-AP

IHC staining of human breast cancer using 15058-1-AP

IHC staining of human breast cancer using 15058-1-AP

IHC staining of mouse brain using 15058-1-AP

IHC staining of human colon using 15058-1-AP

IF Staining of HeLa using 15058-1-AP

FC experiment of HeLa using 15058-1-AP

ARPC2 Polyclonal antibody

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages

Aberrant cortex contractions impact mammalian oocyte quality

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.

WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.

The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.