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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARPC1A Polyclonal antibody (17538-1-AP)

    WB analysis using 17538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17538-1-AP (ARPC1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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ARPC1A Polyclonal antibody

ARPC1A Polyclonal Antibody for WB, ELISA
Cat No. 17538-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Arc40, ARPC1A, SOP2 like protein, SOP2Hs, SOP2L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17538-1-AP targets ARPC1A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARPC1A fusion protein Ag11593 相同性解析による交差性が予測される生物種
Full Name actin related protein 2/3 complex, subunit 1A, 41kDa
Calculated molecular weight 370 aa, 42 kDa
Observed molecular weight 37-42 kDa
GenBank accession numberBC039594
Gene Symbol ARPC1A
Gene ID (NCBI) 10552
RRIDAB_3085527
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARPC1A, also known as SOP2L, belongs to the WD repeat ARPC1 family. ARPC1A is most similar to ARPC1B, and the similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells (PMID: 9230079). ARPC1A functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (PMID: 8978670). Western blot analysis detected ARPC1A at an apparent molecular mass of 37-42 kDa.

Protocols

Product Specific Protocols
WB protocol for ARPC1A antibody 17538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols