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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARP3/ARP3B Polyclonal antibody (13822-1-AP)

    WB analysis using 13822-1-AP (same clone as 13822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using ARP3/ARP3B Polyclonal antibody (13822-1-AP)

    WB analysis of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using ARP3/ARP3B Polyclonal antibody (13822-1-AP)

    WB analysis of human liver using 13822-1-AP (same clone as 13822-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using ARP3/ARP3B Polyclonal antibody (13822-1-AP)

    IP experiment of mouse spleen using 13822-1-AP (same clone as 13822-1-PBS)

    IP result of anti-ARP3/ARP3B (IP:13822-1-AP, 4ug; Detection:13822-1-AP 1:1000) with mouse spleen tissue lysate 4000ug. This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ARP3/ARP3B Polyclonal antibody (13822-1-AP)

    IHC staining of human kidney using 13822-1-AP (same clone as 13822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13822-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARP3/ARP3B Polyclonal antibody, PBS Only

ARP3/ARP3B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13822-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13822-1-PBS targets ARP3/ARP3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4922

Product name: Recombinant human ACTR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-418 aa of BC044590

Sequence: RQVGERTLTGTVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRDREVGIPPEQSLETAKAVKERYSYVCPDLVKEFNKYDTDGSKWIKQYTGINAISKKEFSIDVGYERFLGPEIFFHPEFANPDFTQPISEVVDEVIQNCPIDVRRPLYKNIVLSGGSTMFRDFGRRLQRDLKRTVDARLKLSEELSGGRLKPKPIDVQVITHHMQRYAVWFGGSMLASTPEFYQVCHTKKDYEEIGPSICRHNPVFGVMS

相同性解析による交差性が予測される生物種
Full Name ARP3 actin-related protein 3 homolog (yeast)
Calculated molecular weight 418 aa 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC044590
Gene Symbol ARP3/ACTR3
Gene ID (NCBI) 10096
RRIDAB_2221967
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP61158
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

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