Validation Data Gallery

Filter: AllWBIPIF/ICCFC (Intra)

WB analysis using 14268-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14268-1-AP
WB result of BMAL1 antibody (14268-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BMAL1 transfected HeLa cells.
WB analysis using 14268-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 14268-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 14268-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14268-1-AP
IP result of anti-BMAL1 (IP:14268-1-AP, 4ug; Detection:14268-1-AP 1:300) with HeLa cells lysate 1600ug.
IF Staining of U-87 MG using 14268-1-AP
Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using BMAL1 antibody (14268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
FC experiment of HeLa using 14268-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARNTL (14268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

BMAL1 Polyclonal antibody

BMAL1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14268-1-AP

Publications(72)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

ARNTL, ARNT3, Aryl hydrocarbon receptor nuclear translocator-like protein 1, Basic helix-loop-helix ARNT-like protein 1, Basic-helix-loop-helix-PAS protein MOP3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (72)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-1080 cells, 3T3-L1 cells, HeLa cells, SH-SY5Y cells, mouse liver tissue, MCF-7 cells, NCI-H1299 cells, rat liver tissue
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	U-87 MG cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 12 publications below
WB	See 60 publications below
IHC	See 15 publications below
IF	See 10 publications below
IP	See 1 publications below
CoIP	See 2 publications below
ChIP	See 5 publications below

Product Information

14268-1-AP targets BMAL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5586 Product name: Recombinant human ARNTL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC041129 Sequence: MADQRMDISSTISDFMSPGPTDLLSSSLGTSGVDCNRKRKGSSTDYQESMDTDKDDPHGRLEYTEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLTVLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVSESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDITPGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFPSTCSKKKDRKSFCTIHSTGYLKSWPPTKMGLDEDNEP 相同性解析による交差性が予測される生物種
Full Name	aryl hydrocarbon receptor nuclear translocator-like
Calculated molecular weight	69 kDa
Observed molecular weight	69-75 kDa
GenBank accession number	BC041129
Gene Symbol	ARNTL
Gene ID (NCBI)	406
RRID	AB_2878037
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00327
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

Protocols

Product Specific Protocols
IF protocol for BMAL1 antibody 14268-1-AP	Download protocol
IP protocol for BMAL1 antibody 14268-1-AP	Download protocol
WB protocol for BMAL1 antibody 14268-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis. Authors - Ran Lin KD Validated View Article
human	WB	Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. Authors - Yi Liu View Article
mouse	WB	J Pineal Res Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors Authors - Yao Zuo View Article
	WB	Sci Total Environ Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario Authors - Fangnon Firmin Fangninou View Article
mouse	IHC	Theranostics Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia. Authors - Shuai Wang View Article
mouse	WB	Environ Pollut Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction. Authors - Feifei Shi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14268-1-AP

WB analysis of HeLa using 14268-1-AP

WB analysis using 14268-1-AP

WB analysis of 3T3-L1 using 14268-1-AP

WB analysis of HT-1080 using 14268-1-AP

IP experiment of HeLa using 14268-1-AP

IF Staining of U-87 MG using 14268-1-AP

FC experiment of HeLa using 14268-1-AP

BMAL1 Polyclonal antibody

BMAL1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors

Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario

Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia.

Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.