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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ARNT2 Polyclonal antibody (12810-1-AP)

    WB analysis of Jurkat using 12810-1-AP (same clone as 12810-1-PBS)

    WB result of ARNT2 antibody (12810-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARNT2 transfected Jurkat cells. This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ARNT2 Polyclonal antibody (12810-1-AP)

    WB analysis of HEK-293 using 12810-1-AP (same clone as 12810-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using ARNT2 Polyclonal antibody (12810-1-AP)

    WB analysis of Raji using 12810-1-AP (same clone as 12810-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ARNT2 Polyclonal antibody (12810-1-AP)

    WB analysis of Jurkat using 12810-1-AP (same clone as 12810-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ARNT2 Polyclonal antibody (12810-1-AP)

    WB analysis of HEK-293 using 12810-1-AP (same clone as 12810-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12810-1-AP (ARNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ARNT2 Polyclonal antibody (12810-1-AP)

    IHC staining of human gliomas using 12810-1-AP (same clone as 12810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12810-1-AP (ARNT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ARNT2 Polyclonal antibody (12810-1-AP)

    IHC staining of human gliomas using 12810-1-AP (same clone as 12810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12810-1-AP (ARNT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12810-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ARNT2 Polyclonal antibody, PBS Only

ARNT2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12810-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12810-1-PBS targets ARNT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3536

Product name: Recombinant human ARNT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 363-716 aa of BC036099

Sequence: QDLLGKDILEFCHPEDQSHLRESFQQVVKLKGQVLSVMYRFRTKNREWMLIRTSSFTFQNPYSDEIEYIICTNTNVKQLQQQQAELEVHQRDGLSSYDLSQVPVPNLPAGVHEAGKSVEKADAIFSQERDPRFAEMFAGISASEKKMMSSASAAGTQQIYSQGSPFPSGHSGKAFSSSVVHVPGVNDIQSSSSTGQNMSQISRQLNQSQVAWTGSRPPFPGQQIPSQSSKTQSSPFGIGTSHTYPADPSSYSPLSSPATSSPSGNAYSSLANRTPGFAESGQSSGQFQGRPSEVWSQWQSQHHGQQSGEQHSHQQPGQTEVFQDMLPMPGDPGNEWWSPHSRQHFRQPISYARM

相同性解析による交差性が予測される生物種
Full Name aryl-hydrocarbon receptor nuclear translocator 2
Calculated molecular weight 716 aa, 79 kDa
Observed molecular weight 80-85 kDa
GenBank accession numberBC036099
Gene Symbol ARNT2
Gene ID (NCBI) 9915
RRIDAB_2059456
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBZ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Aryl-hydrocarbon receptor nuclear translocator 2(ARNT2), is a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. It specifically recognizes the xenobiotic response element(XRE). ARNT2 plays an important role in normal glucose handling in pancreatic beta cell function in humans and mice. ARNT2 proteins are dimeric partners for other PAS proteins such as HIF and SIM. ARNT2 and the other ARNT family members, e.g., ARNT, hypoxin inducible factor 1α (HIF1α), HIF2α, and aryl hydrocarbon receptor (AhR), are thought to function as heterodimers, and regulate the expression, and thereby the function, of many genes. Under hypoxic conditions, it complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. Modulation of ARNT2 in pancreatic beta cells affects glucose stimulated INS secretion (GSIS). This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ARNT2.

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