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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    WB analysis using 66732-1-Ig (same clone as 66732-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT/HIF-1 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    IF Staining of A549 using 66732-1-Ig (same clone as 66732-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARNT/HIF-1 beta antibody (66732-1-Ig, Clone: 3B12C5 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ARNT/HIF-1 beta Monoclonal antibody (66732-1-Ig)

    FC experiment of HeLa using 66732-1-Ig (same clone as 66732-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT/HIF-1 beta (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66732-1-PBS in a different storage buffer formulation.

ARNT/HIF-1 beta Monoclonal antibody, PBS Only

ARNT/HIF-1 beta Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66732-1-PBS
Clone No.3B12C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

ARNT, HIF-1 beta, HIF1B, 3B12C5, bHLHe2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66732-1-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5507

Product name: Recombinant human ARNT,HIF1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-348 aa of BC060838

Sequence: MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQQQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSS

相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
RRIDAB_2882082
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP27540
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

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