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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARMC9 Polyclonal antibody (28001-1-AP)

    WB analysis using 28001-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 28001-1-AP (ARMC9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARMC9 Polyclonal antibody (28001-1-AP)

    IF Staining of hTERT-RPE1 using 28001-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARMC9 antibody (28001-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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ARMC9 Polyclonal antibody

ARMC9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28001-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse placenta tissue, mouse retina tissue, rat retina tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28001-1-AP targets ARMC9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARMC9 fusion protein Ag27511 相同性解析による交差性が予測される生物種
Full Name armadillo repeat containing 9
Calculated molecular weight 817 aa, 92 kDa
Observed molecular weight 76-78 kDa
GenBank accession numberBC004514
Gene Symbol ARMC9
Gene ID (NCBI) 80210
RRIDAB_3669633
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3E5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARMC9 (Armadillo Repeat Containing 9) belongs to the ARMC subfamily characterized by a class of tandem repeat proteins with a length of approximately 42 amino acids (PMID: 34912852). ARMC9 localizes to centrioles and the proximal portion of the cilium in mammalian cilia (PMID: 32453716). The variants of ARMC9 lead to Joubert syndrome (JBTS) ,a recessive neurodevelopmental ciliopathy characterized by characterized by hypotonia, ataxia, abnormal eye movements, and variable cognitive impairment(PMID: 28625504; PMID: 32453716). ARMC9 has two isoforms with molecular weights of 92 kDa and 76 kDa.

Protocols

Product Specific Protocols
WB protocol for ARMC9 antibody 28001-1-APDownload protocol
IF protocol for ARMC9 antibody 28001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols