Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, rat testis tissue |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24011-1-AP targets ARMC2 in WB, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21162 Product name: Recombinant human ARMC2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 510-860 aa of BC030603 Sequence: CYALFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSIQTLLSLFQTFHQLDLHSQKPVGQRGEQHRAQRPPSEAEDVLIKLTRVLANIAIHPGVGPVLAANPGIVGLLLTTLEYKSLDDCEELVINATATINNLSYYQVKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGGIKKLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEELALDGSFDPDLKNYHKLHWETEFKPVAQQLLNRIQRHHTFLEPLPIPSF 相同性解析による交差性が予測される生物種 |
| Full Name | armadillo repeat containing 2 |
| Calculated molecular weight | 867 aa, 97 kDa |
| Observed molecular weight | 78 kDa,97 kDa |
| GenBank accession number | BC030603 |
| Gene Symbol | ARMC2 |
| Gene ID (NCBI) | 84071 |
| RRID | AB_3669428 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8NEN0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ARMC2 protein (Armadillo Repeat Containing 2) is an evolutionarily conserved axonemal-related protein that plays a crucial role in the structure and function of cilia and flagella. It interacts with the kinesin-2 complex through its unique Armadillo repeat domain and acts as an adapter for the flagellar transport (IFT) process, responsible for transporting specific cargoes (such as the precursor proteins necessary for the construction of flagellar axons) from the cell body to the flagellar tip, which is essential for the assembly, maintenance, and signal transduction functions of the flagella. Its dysfunction is directly related to abnormal sperm flagellar morphology and movement disorders.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARMC2 antibody 24011-1-AP | Download protocol |
| WB protocol for ARMC2 antibody 24011-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |