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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A/ARL8B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis of human placenta using 68186-1-Ig (same clone as 68186-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    WB analysis using 68186-1-Ig (same clone as 68186-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68186-1-Ig (ARL8A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of C6 using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of Neuro-2a using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of A549 using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ARL8A/ARL8B Monoclonal antibody (68186-1-Ig)

    IF Staining of HEK-293 using 68186-1-Ig (same clone as 68186-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ARL8A/ARL8B antibody (68186-1-Ig, Clone: 2F4C3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68186-1-PBS in a different storage buffer formulation.

ARL8A/ARL8B Monoclonal antibody, PBS Only

ARL8A/ARL8B Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68186-1-PBS
Clone No.2F4C3

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARL8A, GIE2, ARL10B, ADP-ribosylation factor-like protein 8A, ADP-ribosylation factor-like protein 10B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68186-1-PBS targets ARL8A/ARL8B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10758

Product name: Recombinant human ARL8A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-186 aa of BC015408

Sequence: MIALFNKLLDWFKALFWKEEMELTLVGLQYSGKTTFVNVIASGQFNEDMIPTVGFNMRKITKGNVTIKLWDIGGQPRFRSMWERYCRGVSAIVYMVDAADQEKIEASKNELHNLLDKPQLQGIPVLVLGNKRDLPGALDEKELIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 8A
Calculated molecular weight 186 aa, 21 kDa
Observed molecular weight18 kDa, 21 kDa
GenBank accession numberBC015408
Gene Symbol ARL8A
Gene ID (NCBI) 127829
RRIDAB_3085047
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96BM9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ARL8A (ADP-ribosylation factor-like protein 8A) is also named as ARL10B, GIE2 and belongs to the small GTPase superfamily. ARL8A is localized to lysosomes in mammalian cells (PMID: 16537643). In neurons, ARL8A mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles that are required for presynaptic biogenesis and synaptic function. ARL8A is upregulated in many cancers such as endometrial cancer (PMID: 32070877), colorectal cancer (PMID: 30546056) and inflammatory breast cancer (PMID: 27648361), and has been associated with poor survival outcomes. ARL8A/ARL8B can be recognized by this antibody.

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