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ARL13B Polyclonal antibody
ARL13B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 17711-1-AP
Validation Data Gallery
|Positive WB detected in||L02 cells, NIH/3T3 cells, mouse liver tissue, mouse kidney tissue, mouse brain tissue, rat liver tissue, rat kidney tissue|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||mouse brain tissue, human testis tissue, human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells, hTERT-RPE1 cells, hTERT-RPE1 cells, NIH3T3 cells, mouse embryonic fibroblasts|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17711-1-AP targets ARL13B in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, chicken, rat, sheep, mouse, monkey, zebrafish, pig, Xenopus, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARL13B fusion protein Ag12015 相同性解析による交差性が予測される生物種|
|Full Name||ADP-ribosylation factor-like 13B|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||40-48 kDa, 60 kDa|
|GenBank accession number||BC094725|
|Gene ID (NCBI)||200894|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two speciﬁc bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
|Product Specific Protocols|
|WB protocol for ARL13B antibody 17711-1-AP||Download protocol|
|IHC protocol for ARL13B antibody 17711-1-AP||Download protocol|
|IF protocol for ARL13B antibody 17711-1-AP||Download protocol|
|IP protocol for ARL13B antibody 17711-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gagan (Verified Customer) (07-03-2023)
Antibody gives good staining for cilia.
Mi (Verified Customer) (05-29-2023)
An excellent antibody, it works pretty well in human white adipocytes!
Elisa (Verified Customer) (03-17-2023)
Great antibody for centrosome and cilia visualizationin RPE1 cells. Cells were fixed in cold methanol for 10' at -20C, rehydrated with PBS for 5', and permeabilized with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature). Image show Arl13b (in magenta), DNA (Hoechst in blue) and g-tubulin (in green).
Serhiy (Verified Customer) (12-21-2022)
Excellent for immunofluorescent staining of ciliary Arl13b in mouse cells. Image shows ciliary axonemes immunostained for Arl13b (red).
Kaviya (Verified Customer) (09-19-2022)
I use this antibody for IHC on brain tissue sections and it generally works well.
Charlotte (Verified Customer) (07-29-2022)
NIH-3T3 stained in DAPI (nucleus), FITC (gamma tubulin), Cy3 (Arl13b). The last picture is the merged channels (FITC and Cy3). On the merged channel, we can conclude that all Cy3 signal is validated by the FITC one (gamma tubulin is present at the base of the primary cilia). Perfect antibody !
Sarah (Verified Customer) (02-16-2021)
Works beautifully in all cell types I've tried. Specifically great to see it work in primary cells (glioblastoma, pictured) as well as iPSCs
deng (Verified Customer) (01-30-2020)
Very good for cilia staining
Gabriela (Verified Customer) (01-02-2020)
Very specific antibody for primary cilia. We use it in chicken embryos spinal cord.
KARTHIKEYAN (Verified Customer) (11-20-2019)
This antibody works really well in human and mouse cell lines and in zebrafish samples, we used this antibody for WB, IF and Flow cytometry.
Rebeca (Verified Customer) (09-26-2019)
I have used this antibody for several immunofluorescence stainings of primary cilia. The antibody works beautifully, would definitely recommend!
Pavel (Verified Customer) (09-18-2019)
Strong ciliary staining in MDCK cells. Works both with PFA and MeOH fixation.
Lindsey (Verified Customer) (02-04-2019)
Excellent specificity with minimal background/nonspecific binding; This antibody is ideal because it works just as well in embryonic mouse tissue as it does in adult. I couldn't recommend this product more!
Kishor (Verified Customer) (01-30-2019)
I am very much satisfied with this produced, working good for WB (1:1000) and for IF (1:200)