• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis using 17711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis of mouse liver using 17711-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis of L02 using 17711-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis of mouse kidney using 17711-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis using 17711-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis of NIH/3T3 using 17711-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ARL13B Polyclonal antibody (17711-1-AP)

    WB analysis of mouse brain using 17711-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17711-1-AP (ARL13B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of L02 cells using ARL13B Polyclonal antibody (17711-1-AP)

    IP experiment of L02 using 17711-1-AP

    IP result of anti-ARL13B (IP:17711-1-AP, 3ug; Detection:17711-1-AP 1:1000) with L02 cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IHC staining of mouse brain using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IHC staining of mouse brain using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17711-1-AP (ARL13B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IHC staining of human testis using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IHC staining of human testis using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17711-1-AP (ARL13B Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IHC staining of human liver using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IHC staining of human liver using 17711-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17711-1-AP (ARL13B antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of mouse pancreas using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CoraLite® Plus 488 E-cadherin antibody (CL488-20874, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of MDCK using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of NIH3T3 cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of NIH3T3 cells using 17711-1-AP

    IF result of anti-ARL13B (17711-1-AP) in NIH3T3 cell by Dr. Sudipto.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of hTERT-RPE1 using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (17711-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of MDCK using 17711-1-AP

    Immunofluorescent images of MDCK cells stained with ARL13B rabbit pAb (17711-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure-Goat anti-Rabbit IgG(H+L) for 17711-1-AP, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 66200-1-Ig.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of MDCK using 17711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 17711-1-AP (ARL13B antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of hTERT-RPE1 using 17711-1-AP

    IF result of ARL13B antibody (17711-1-AP) in hTERT-RPE cell fixed with 4% PFA or Methanol.

  • Immunofluorescence (IF) / fluorescent staining of mouse embryonic fibroblasts using ARL13B Polyclonal antibody (17711-1-AP)

    IF Staining of mouse embryonic fibroblasts using 17711-1-AP

    IF result from Dr. Corbit, Kevin. anti-ARL13B (17711-1-AP) mark the cilium of Mouse embryonic fibroblasts.

  • Featured Product
  • KD/KO Validated

ARL13BA Polyclonal antibody

ARL13BA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17711-1-AP
Publications(866)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ARL13B, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2 like protein 1, ARL2L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue, rat kidney tissue
Positive IP detected inL02 cells
Positive IHC detected inmouse brain tissue, human liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, NIH3T3 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17711-1-AP targets ARL13BA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, canine, monkey, chicken, zebrafish, sheep, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12015

Product name: Recombinant human ARL13B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-321 aa of BC094725

Sequence: MFSLMASCCGWFKRWREPVRLANKQDKEGALGEADVIECLSLEKLVNEHKCLCQIEPCSAISGYGKKIDKSIKKGLYWLLHVIARDFDALNERIQKETTEQRALEEQEKQERAERVRKLREERKQNEQEQAELDGTSGLAELDPEPTNPFQPIASVIIENEGKLEREKKNQKMEKDSDGCHLKHKMEHEQIETQGQVNHNGQKNNEFGLVENYKEALTQQLKNEDETDRPSLESANGKKKTKKLRMKRNHRVEPLNIDDCAPESPTPPPPPPPVGWGTPKVTRLPKLEPLGETHHNDFYRKPLPPLAVPQRPNSDAHDVIS

相同性解析による交差性が予測される生物種
Full Name ADP-ribosylation factor-like 13B
Calculated molecular weight 48 kDa
Observed molecular weight 40-48 kDa, 60 kDa
GenBank accession numberBC094725
Gene Symbol ARL13B
Gene ID (NCBI) 200894
RRIDAB_2060867
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SXY8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARL13B, also named ARL2L1, is the Ras superfamily's small ciliary G protein. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B cause Joubert syndrome (JS), an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band will likely represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).

Protocols

Product Specific Protocols
WB protocol for ARL13BA antibody 17711-1-APDownload protocol
IHC protocol for ARL13BA antibody 17711-1-APDownload protocol
IF protocol for ARL13BA antibody 17711-1-APDownload protocol
IP protocol for ARL13BA antibody 17711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

The developmental basis of fingerprint pattern formation and variation

Authors - James D Glover
View Article
humanIF

Nat Biotechnol

Guided self-organization and cortical plate formation in human brain organoids.

Authors - Madeline A Lancaster
View Article
mouseIF

Nature

Quantitative lineage analysis identifies a hepato-pancreato-biliary progenitor niche.

Authors - David Willnow
View Article
mouseIF

Science

Control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium.

Authors - Avishag Mytlis
View Article
humanIF

Nature

A slow-cycling LGR5 tumour population mediates basal cell carcinoma relapse after therapy.

Authors - Adriana Sánchez-Danés
View Article
mouseIF

Nature

Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.

Authors - Matthew B Johnson
View Article