Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
14949-1-AP targets ARIH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6760 Product name: Recombinant human ARIH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 209-507 aa of BC051877 Sequence: MQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKKWIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRYNEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAVDVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSG 相同性解析による交差性が予測される生物種 |
| Full Name | ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC051877 |
| Gene Symbol | ARIH1 |
| Gene ID (NCBI) | 25820 |
| RRID | AB_3085447 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4X5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARIH1 (Ariadne-1 homolog) is a member of the Ariadne family of E3 ubiquitin ligases with a cognate E2 enzyme UBCH79,10,11. ARIH1 plays a role in protein translation regulation in response to DNA damage and to ubiquitinate EIF4E2 (PMID: 15236971, PMID: 1532592, PMID: 24076655, PMID: 27565346, PMID: 23707686). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation (PMID: 33879767).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARIH1 antibody 14949-1-AP | Download protocol |
| WB protocol for ARIH1 antibody 14949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancers (Basel) ARIH1 Inhibition Promotes Microtubule Stability and Sensitizes Breast Cancer Cells to Microtubule-Stabilizing Agents
| ||
Cell Rep HERC5-catalyzed ISGylation potentiates cGAS-mediated innate immunity
| ||
bioRxiv MDA5 ISGylation is crucial for immune signaling to control viral replication and pathogenesis
|