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Validation Data Gallery

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  • Western Blot (WB) analysis of T-47D cells using ARID4B Monoclonal antibody (67384-1-Ig)

    WB analysis of T-47D using 67384-1-Ig (same clone as 67384-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67384-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-231 cells using ARID4B Monoclonal antibody (67384-1-Ig)

    WB analysis of MDA-MB-231 using 67384-1-Ig (same clone as 67384-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARID4B Monoclonal antibody (67384-1-Ig)

    IHC staining of human breast cancer using 67384-1-Ig (same clone as 67384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67384-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARID4B Monoclonal antibody (67384-1-Ig)

    IHC staining of human breast cancer using 67384-1-Ig (same clone as 67384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67384-1-PBS in a different storage buffer formulation.

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ARID4B Monoclonal antibody, PBS Only

ARID4B Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67384-1-PBS
Clone No.1H3E2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

ARID4B, BRCAA1, DKFZp313M2420, RBBP1L1, RBP1L1, SAP180

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67384-1-PBS targets ARID4B in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARID4B fusion protein Ag21485 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 4B (RBP1-like)
Calculated molecular weight 1312 aa, 148 kDa
GenBank accession numberBC130418
Gene Symbol ARID4B
Gene ID (NCBI) 51742
RRIDAB_2918489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ4LE39
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.