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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARID1B Recombinant antibody (82979-6-RR)

    IF Staining of HeLa using 82979-6-RR (same clone as 82979-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1B antibody (82979-6-RR, Clone: 230222A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using ARID1B Recombinant antibody (82979-6-RR)

    FC experiment of SH-SY5Y using 82979-6-RR (same clone as 82979-6-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug ARID1B Recombinant antibody (82979-6-RR, Clone:230222A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82979-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82979-6-RR

    Affinity and Kinetic Characterization of 82979-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82979-6-RR against Human ARID1B were performed. The affinity constant is 1.14 nM.

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ARID1B Recombinant antibody, PBS Only

ARID1B Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82979-6-PBS
Clone No.230222A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

hOsa2, ELD/OSA1, DAN15, BRIGHT, BAF250b

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82979-6-PBS targets ARID1B in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARID1B fusion protein Ag30643 相同性解析による交差性が予測される生物種
Full Name AT rich interactive domain 1B (SWI1-like)
Calculated molecular weight 236 kDa
GenBank accession numberNM_017519
Gene Symbol ARID1B
Gene ID (NCBI) 57492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NFD5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.