Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of PC-12 using 14092-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Transfected HEK-293 using 14092-1-AP
WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.
WB analysis of NIH/3T3 using 14092-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14092-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 14092-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14092-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14092-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14092-1-AP
IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.
IHC staining of mouse brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 14092-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14092-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ARHGEF7 Polyclonal antibody

ARHGEF7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14092-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIAA0142, COOL-1, COOL1, COOL 1, Beta-Pix

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-12 cells, mouse skeletal muscle tissue, K-562 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, Transfected HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human rectal cancer tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

14092-1-AP targets ARHGEF7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5223 Product name: Recombinant human ARHGEF7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 455-803 aa of BC060776 Sequence: KELELQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPTTGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLPSHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADDDQPSLDSLGRRSSLSRLEPSDLSEDSDYDSIWTAHSYRMGSTSRKSCCSYISHQN 相同性解析による交差性が予測される生物種
Full Name	Rho guanine nucleotide exchange factor (GEF) 7
Calculated molecular weight	803 aa, 90 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC060776
Gene Symbol	ARHGEF7
Gene ID (NCBI)	8874
RRID	AB_2227567
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14155
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for ARHGEF7 antibody 14092-1-AP	Download protocol
IHC protocol for ARHGEF7 antibody 14092-1-AP	Download protocol
IP protocol for ARHGEF7 antibody 14092-1-AP	Download protocol
WB protocol for ARHGEF7 antibody 14092-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7. Authors - Ruth Chia View Article
human	WB,IHC	Am J Transl Res Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3. Authors - Tao Ma View Article
rat	WB	J Control Release Dentin tubules as a long-term sustained release carrier to accelerate bone repair by loading FTY720 Authors - Jiaman Xie View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of PC-12 using 14092-1-AP

WB analysis of Transfected HEK-293 using 14092-1-AP

WB analysis of NIH/3T3 using 14092-1-AP

WB analysis of K-562 using 14092-1-AP

WB analysis of mouse skeletal muscle using 14092-1-AP

WB analysis of Jurkat using 14092-1-AP

WB analysis of HeLa using 14092-1-AP

IP experiment of HeLa using 14092-1-AP

IHC staining of mouse brain using 14092-1-AP

IHC staining of mouse brain using 14092-1-AP

IHC staining of rat brain using 14092-1-AP

IHC staining of rat brain using 14092-1-AP

IHC staining of human rectal cancer using 14092-1-AP