ARHGEF5 Polyclonal antibody

Name: ARHGEF5 Polyclonal antibody
Brand: Proteintech
SKU: 11379-1-AP

ARHGEF5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11379-1-AP

Synonyms

ARHGEF5, GEF5, Oncogene TIM, P60, p60 TIM, TIM, TIM1

Validation Data Gallery

Applications:

AllWBIPIHCIF

WB analysis of MCF-7 using 11379-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11379-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11379-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11379-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11379-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11379-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 11379-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 11379-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 11379-1-AP
IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
IHC staining of human lung cancer using 11379-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARHGEF5 antibody included
IHC staining of human lung cancer using 11379-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARHGEF5 antibody included
IF Staining of HeLa using 11379-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11379-1-AP (ARHGEF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 11379-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF7 cells, HEK-293 cells, HeLa cells, human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ARHGEF5 Ready-To-Use IHC Kit. ARHGEF5 primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11379-1-AP targets ARHGEF5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARHGEF5 fusion protein Ag1892 相同性解析による交差性が予測される生物種
Full Name	Rho guanine nucleotide exchange factor (GEF) 5
Calculated molecular weight	177 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC010046
Gene symbol	ARHGEF5
Gene ID (NCBI)	7984
RRID	AB_2060050
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGEF5 antibody 11379-1-AP	Download protocol
IHC protocol for ARHGEF5 antibody 11379-1-AP	Download protocol
IF protocol for ARHGEF5 antibody 11379-1-AP	Download protocol
IP protocol for ARHGEF5 antibody 11379-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB, IF	J Biol Chem Odontogenic ameloblast-associated protein (ODAM) Mediates Junctional Epithelium Attachment to Tooth via Integrin-ODAM-Rho guanine nucleotide exchange factor 5 (ARHGEF5)-Ras homolog gene family member A (RhoA) Signaling. Authors - Hye-Kyung Lee View Article
human	IHC	Oncol Rep Co-expression of Rho guanine nucleotide exchange factor 5 and Src associates with poor prognosis of patients with resected non-small cell lung cancer. Authors - He Ping P View Article
human	WB	Cell Biochem Funct ODAM inhibits RhoA-dependent invasion in breast cancer. Authors - Hye-Kyung Lee View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARHGEF5 Polyclonal antibody

ARHGEF5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 11379-1-AP

WB analysis of human kidney using 11379-1-AP

WB analysis of HeLa using 11379-1-AP

WB analysis of human liver using 11379-1-AP

WB analysis of HEK-293 using 11379-1-AP

WB analysis of mouse liver using 11379-1-AP

WB analysis of rat kidney using 11379-1-AP

WB analysis of mouse kidney using 11379-1-AP

IP experiment of mouse kidney using 11379-1-AP

IHC staining of human lung cancer using 11379-1-AP

IHC staining of human lung cancer using 11379-1-AP

IF Staining of HeLa using 11379-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Odontogenic ameloblast-associated protein (ODAM) Mediates Junctional Epithelium Attachment to Tooth via Integrin-ODAM-Rho guanine nucleotide exchange factor 5 (ARHGEF5)-Ras homolog gene family member A (RhoA) Signaling.

Co-expression of Rho guanine nucleotide exchange factor 5 and Src associates with poor prognosis of patients with resected non-small cell lung cancer.

ODAM inhibits RhoA-dependent invasion in breast cancer.